phusion dna

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  Phusion® High-Fidelity DNA Polymerase NEB

  Phusion DNA Polymerase is an ideal choice for cloning and can be used for long or difficult amplicons. With an error rate > 50-fold lower than that of Taq DNA Polymerase and 6-fold lower than that of Pyrococcus furiosus DNA Polymerase (1), Phusion is one

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  Phusion DNA PolymeraseNew England Biolabs

  Phusion® DNA Polymerase was developed by Finnzymes Oy, now a part of Thermo Fisher Scientific. This product is manufactured by New England Biolabs, Inc. under agreement with, and under the performance specifications of Thermo Fisher Scientific. Phusion® is a registered trademark and property of Thermo Fisher Scientific.

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  PhusionOpenWetWare

   · add to 50μl. Phusion DNA polymerase (2U/μ) 0.5μl. 0.02 U/pl. A 2x supermix is now available containing either HF buffer or GC buffer, dNTPs, and Phusion polymerase. See references section below for links.

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  PhusionDNA Thermo Fisher

  Phusion Green High-Fidelity DNA Polymerase (2 U/µL) 100 U ¥770 ¥539 F534L Phusion Green High-Fidelity DNA Polymerase (2 U/µL) 500 U ¥3,058 ¥2,141 F531S Phusion High-Fidelity PCR Master Mix with HF Buffer 100 x 50 μL rxns ¥1,243 ¥870 F531L

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  Phusion® High-Fidelity DNA Polymerases NEB

  DNA polymerases with high fidelity are important for applications in which the DNA sequence needs to be correct after amplification. Manufactured and quality-controlled at New England Biolabs, Thermo Scientific ® Phusion ® High-Fidelity DNA Polymerase offers both high fidelity and robust performance, and thus can be used for all PCR applications. Its unique structure, a novel Pyrococcus-like

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  Thermo Scientific Phusion High–Fidelity DNA Polymerase

   · • The Phusion ™ High–Fidelity DNA Polymerase should be pipetted carefully and gently as the high glycerol content (50%) in the storage buffer may otherwise lead to pipetting errors. • Due to the nature of the Phusion ™ High–Fidelity DNA Polymerase, the optimal reaction conditions may differ from PCR protocols for standard DNA

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  Phusion DNA(Phusion DNA Polymerase)

   · Phusion DNA (Phusion DNA Polymerase) Phusion DNA Polymerase,。Pfu DNADNASso7d,

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  Phusion™ Plus Green PCR Master Mix/Phusion™

   · Phusion Plus DNA Polymerase is a hot-start, high-fidelity DNA polymerase that brings together protein fusion technology and universal primer annealing. It is a Pyrococcus-like proofreading DNA polymerase fused to a processivity-enhancing domain, enabling Phusion Plus Green PCR Master Mix to generate PCR sequences with high accuracy, sensitivity, and inhibitor tolerance.

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  Phusion U DNA Thermo Fisher

  Phusion U PCR （F-533S F-533L） 2X ，。。Phusion U Green （F-556S F-556L） Phusion U DNA 5X Phusion Green 。

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  Phusion™ High-Fidelity DNA Polymerase from Finnzymes Oy

  The Phusion DNA Polymerase generates PCR products with accuracy and speed previously unattainable with a single enzyme, even on your most difficult templates. The unique structure and characteristics of Phusion DNA Polymerase make it a superior choice for cloning and set a

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  Phusion™ High-Fidelity DNA Polymerase (2 U/µL)

  Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR. Featuring an error rate 50-fold lower than that of Taq, and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is excellent choice for cloning and other applications requiring high fidelity. Phusion DNA Polymerases offer robust performance with short protocol times, even in the presence

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  Phusion DNA PolymeraseNew England Biolabs GmbH

  Phusion® Technology. Phusion DNA Polymerase brings together a novel Pyrococcus-like enzyme with a processivity-enhancing domain and generates PCR products with accuracy and speed previously unattainable with a single enzyme, even on your most difficult templates. Additionally, Phusion DNA Polymerase is capable of amplifying long templates. In the quality control tests of Phusion DNA

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  LabAid Phusion High-Fidelity DNA Polymerases

   · Phusion DNA polymerase 0.5 μL 0.2 μL 0.02 U/μL Water To 50 μL total To 20 μL total* If you are using any of the Phusion PCR master mix products, add 25 or 10 μL of the 2X master mix (depending on the final reaction volume).

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  Phusion High Fidelity DNA Polymerases Flyer

   · unique fusion technique, Phusion DNA polymerases generate PCR products with very high accuracy and speed. In addition, Phusion DNA polymerases are tolerant of various inhibitors, allowing for robust amplifi cation with minimal optimization. The processivity of Phusion DNA polymerases is ~10-fold greater than that of Pfu DNA polymerase and twice

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  Phusion™ DNA (2 U/µL)Thermo

  Thermo Scientific Phusion DNA PCR “”。Phusion DNA Taq 50、 Pfu 6，。 DNA ，Phusion DNA ，

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  Phusion Site-Directed Mutagenesis Kit User Guide

   · The Phusion™ DNA polymerase ensures high fidelity for the exponential amplification, thus reducing unwanted secondary mutations and enabling amplification of large plasmids up to 10 kb. A hot start modification in the Phusion™ DNA polymerase combines the DNA polymerase

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  Phusion™ High-Fidelity DNA Polymerase

   · Phusion DNA polymerase gave strong specific bands even with the shortest extension time, completing the 3.8 kb fragment with only a one minute combined annealing and extension step. Enzyme amounts also indicate that significantly less of the highly processive Phusion DNA polymerase is required to complete the task.

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  Thermo Scientific Phusion High-Fidelity DNA Polymerase

   · Phusion DNA Polymerase should be pipetted carefully and gently as the high glycerol content (50%) in the storage buffer may otherwise lead to pipetting errors. It is critical that the Phusion DNA Polymerase is the last component added to the PCR mixture, since the enzyme exhibits 3´→5´ absence of dNTPs.Due to the nature of Phusion DNA

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  PCR amplification of plasmid DNA (Phusion) · Benchling

  PCR amplification of plasmid DNA (Phusion) Updated 3/8/2019 7 43pm. Step-by-step. by SoundBio iGEM (created by Yoshi Goto) Introduction. This protocol describes the method used for the amplification of specific DNA fragments by the Polymerase Chain Reaction, using plasmid DNA as a template. The amplified products may subsequently be analysed

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  Phusion Plus DNA PolymeraseThermo Fisher Scientific

   · Phusion™ Plus DNA Polymerase 50 µL 250 µL 4 × 250 µL –25°C to –15°C Phusion™ Plus Buffer 1.25 mL 5 × 1.25 mL 20 × 1.25 mL Phusion™ GC Enhancer 1.25 mL 4 × 1.25 mL 16 × 1.25 mL General guidelines • Use 98°C for denaturation. • Use 15–30 s/kb for extension. •

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  DNA Polymerase Selection Chart NEB

  DNA Polymerase Selection Chart. The following table lists properties that should be considered when choosing a polymerase. Since these properties can depend on reaction conditions, the primary references should be consulted prior to use in a given application. PCR

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  PCR Protocol Phusion® DNA Polymerase NEB

  Phusion DNA Polymerase may be diluted in 1X HF or GC Buffer just prior to use in order to reduce pipetting errors. Please note that protocols with Phusion DNA Polymerase may differ from protocols with other standard polymerases. As such, conditions recommended below

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  Guidelines for PCR Optimization with Phusion High-Fidelity

   · The Phusion DNA Polymerase has the ability to stablize primer-template hybridization. As a basic rule, for primers >20 nt, anneal for 10-30 seconds at Tm 3°C of the lower Tm primer. The Tm’s should be calculated with the nearest neighbor method as results from primer Tm calculations can vary significantly depending on the method used.

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  Taq Thermo Scientific Phusion DNA Polymerases

   · Phusion DNA Polymerases are an ideal choice for amplification of long templates. The high enzyme processivity allows amplification of a wide variety of template sizes. Amplicons up to 20 kb are produced with high yields, short cycling times, and high fidelity. Fast PCR with high fidelity Phusion DNA Polymerases incorporate a high number of

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  PCR Reaction PCR Conditions For Phusion DNA

   · For Phusion DNA Polymerase (Thermo Fisher) Standard 5X Phusion HF buffer *10 ul 98°C1 min 10mM dNTP1 ul 98°C15 s Primers/each (10µM)2.5 ul 55°C20 s 30 cycles cDNA OR0.5 ul 72°C –* Genomic DNA OR0.5 ul 72°C4 min Plasmid DNA1.0 ul 4°Cfor ever

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  Phusion™ Plus DNA Polymerase/Phusion™ Plus

   · Thermo Scientific Phusion Plus DNA Polymerase is a hot-start, high-fidelity DNA polymerase that brings together protein fusion technology and universal primer annealing. It is a Pyrococcus-like proofreading DNA polymerase fused to a processivity-enhancing domain, enabling Phusion Plus DNA Polymerase to generate PCR sequences with high accuracy, sensitivity, and inhibitor tolerance.

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  Thermo Scientific Phusion High-Fidelity DNA Polymerase

   · The Phusion DNA Polymerase generates long templates with an accuracy and speed previously unattainable with a single enzyme, even on the most difficult templates. The extreme fidelity makes Phusion DNA Polymerase a superior choice for cloning. Using a lacI-based method modified from previous studies1, the error rate of Phusion DNA Polymerase in

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  Phusion® High-Fidelity PCR Kit NEB

  Phusion® DNA Polymerase was developed by Finnzymes Oy, now a part of Thermo Fisher Scientific. This product is manufactured by New England Biolabs, Inc. under agreement with, and under the performance specifications of Thermo Fisher Scientific. Phusion® is a registered trademark and property of Thermo Fisher Scientific.

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  DNA Polymerase Selection Chart NEB

  DNA Polymerase Selection Chart. The following table lists properties that should be considered when choosing a polymerase. Since these properties can depend on reaction conditions, the primary references should be consulted prior to use in a given application. PCR

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  Phusion® High-Fidelity DNA Polymerases Products NEB

  Phusion® High-Fidelity PCR Kit. Based on your Freezer Program type, you are trying to add a product to your cart that is either not allowed or not allowed with the existing contents of your cart. Please review and update your order accordingly If you have any questions, please contact Customer Service at [email protected] or x 8.

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  PhusionIIDNA.PDFBOOK118

   · PhusionIIDNA.PDF, • 50μl Phusion DNA 0.5‐1.0U，2U/50μl （4.1 ）。 • 15‐30s/kb ， Phusion®II DNA 1min

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  APExBIOPhusion high-fidelity

  PhusionDNAPyrococcusDNA。，Phusion DNAPCR。，Phusion DNA，。

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  Frontiers Insertion of Badnaviral DNA in the Late Blight

   · PCRs were done using Phusion DNA polymerase (NEB, Ipswich, MA, United States). PCR mixes with a final volume of 25 μl contained 2.5 μl of 10 × GC buffer, 0.2 μl of 25 mM MgCl 2, 1.6 μl of 2.5 mM dNTPs, 1 U of 5,000 U/ml Phusion DNA polymerase and 5 μl of each primer at a concentration of 5 μM. Thermocycling conditions were an initial

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  Phusion® High-Fidelity DNA Polymerase New England

  Phusion DNA Polymerase possesses 5´→ 3´ polymerase activity, 3´→ 5´ exonuclease activity and will generate blunt-ended products. Phusion DNA Polymerase is supplied with standard 5X Phusion HF Buffer, as well as 5X Phusion GC Buffer, which can be used for complex or GC-rich templates.

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  Phusion II DNA (2 U/µL)Thermo

  Thermo Scientific Phusion DNA PCR “”。Phusion DNA Taq 50、 Pfu 6，。 DNA ，Phusion DNA ，

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  Phusion High-Fidelity DNA PolymeraseSevier Lab

   · Phusion DNA Polymerase in Phusion HF Buffer is determined to be 4.4 x 10-7, which is approximately 50-fold lower than that of Thermus aquaticus DNA polymerase, and 6-fold lower than that of Pyrococcus furiosus DNA polymerase. Phusion DNA Polymerase possesses the following activities 5´→3´ DNA polymerase activity and 3´→5´ exonuclease